A convenient package of tools that allows the experimenter to measure general acetylation of histone H4.
The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric) is suitable for specifically measuring total histone H4 acetylation using a variety of mammalian cells (human, mouse, etc.), including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:
• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.
• Captures histone H4 acetylated at any lysine site with the detection limit as low as 0.4 ng/well and detection range from 5 ng-2 ug/well of histone extracts.
• The control is conveniently included for the quantification of the amount of acetylated histone H4.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.