A complete set of essential components which enables the experimenter to detect if histone H4 is acetylated and quantify the amount of the acetylated histone H4.
The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric) is suitable for specifically detecting acetylated histone H4 and quantifying the amount of acetylated histone H4 using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, and cultured adherent and suspension cells. The kit has the following advantages:
• Quick and efficient procedure, which can be finished within 2.5 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography.
• Captures histone H4 acetylated at any lysine site with the detection limit as low 2 ng/well and detection range from 5 ng-2 ug/well of histone extracts.
• The control is conveniently included for the quantification of the amount of acetylated histone H4.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.