A complete set of optimized buffers and reagents that allows the experimenter to colorimetrically measure DNA methyltransferase activity or inhibition at extremely fast speeds on a 96-stripwell microplate.
The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik DNMT Activity/Inhibition Assay Ultra Kit is a further refinement of its popular predecessor kit by enhancing sample signals and significantly minimizing background signals, in addition to being five times more sensitive.
The kit has the following advantages and features:
• Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes.
• Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
• The ultra-sensitive detection limit can be as low as 0.5 ug of nuclear extract or 0.5 ng of purified enzymes, which is five times better than the predecessor kit.
• optimized antibody & enhancer solutions allow high specificity to 5-mC without cross-reactivity to unmethylated cytosine.
• Strip-well microplate format allows for either low or high throughput analysis.