A complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from various mammalian tissues.
In the assay, an antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR, and southern blot as well as DNA microarray.
The kit has the following advantages:
• Directly immunoprecipitate methylated fractions of DNA from tissue lysates.
• Highly efficient enrichment of methylated DNA: > 95%
• The fastest procedure available, which can be finished within 3 and a half hours.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included: save time and reduce labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.