A complete set of essential components which enables an experimenter to fluorometrically quantify levels of histone H4 proteins independent of their modified states.
It can also be used for normalizing the modified histone H4 content of samples when run in parallel with Epigentek's histone modification quantification kit series. The EpiQuik Total Histone H4 Quantification Kit (Fluorometric) is suitable for specifically measuring total histone H4 using human, mouse, and rat samples, including fresh and frozen tissues and cultured adherent and suspension cells.
• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative fluorometric assay without the need for radioactivity, electrophoresis, or chromatography.
• Specifically captures histone H4 with the detection limit as low as 0.2 ng/well and detection range from 50 ng to 500 ng/well of histone extracts.
• The unmodified histone H4 control is conveniently included for the quantification of the amount of total histone H4.
• Strip microplate format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.