A complete set of optimized buffers and reagents to colorimetrically quantify N6-methyladenosine (m6A or 6mA) in RNA.
It is suitable for a direct detection of m6A RNA methylation status using total RNA isolated from any species such as mammals, plants, fungi, bacteria, and viruses.
The kit has the following advantages and features:
• Colorimetric ELISA-like assay using an m6A antibody with easy-to-follow steps for convenience and speed. The entire procedure can be completed within 3 hours and 45 minutes.
• High sensitivity, of which the detection limit can be as low as 10 pg of m6A.
• Unique binding solution allows RNA >70 nts to be tightly bound to the wells, which enables quantification of m6A from both mRNA and ncRNA such as tRNA, rRNA, and snRNA.
• optimized antibody and enhancer solutions allow high specificity to m6A, with no cross-reactivity to unmethylated adenosine within the indicated concentration range of the sample RNA.
• Universal positive and negative controls are included, which are suitable for quantifying m6A from any species.
• Strip-well microplate format makes the assay flexible for manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.