A complete set of optimized reagents, designed for an easy and fast fluorometric measurement of LSD1 activity or inhibition.
The antibody-based, immunospecific method directly detects LSD1-converted demethylated products, rather than by- products, in a 96 stripwell microplate format.
• 3 hour procedure in a 96 stripwell microplate format allows for either manual or high throughput analysis.
• Directly measures LSD1 activity via a straightforward detection of LSD1-converted demethylated products, rather than by-products, thus eliminating assay interferences caused by thiol-containing chemicals such as DTT, GSH, and 2-mercaptoethanol.
• Both cell/tissue extracts and purified LSD1 can be used, which allows for the detection of inhibitory effects of LSD1 inhibitors in vivo and in vitro.
• Sensitivity is up to 50 times higher than H2o2/formaldehyde release-based LSD1 assays, allowing activity to be fluorometrically detected from as low as 2 ng of purified LSD1 enzyme.
• Demethylated H3-K4 standard is included, allowing specific activity of LSD1 to be quantified.
• Accurate, reliable, and consistent with extremely low background signals.