A complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format, followed by a shearing step by sonication. 100 Preperations.
Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash one-Step ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
When performing ChIP, chromatin or DNA-protein complex in cells or tissues should first be isolated and sheared to a desired size. The ChromaFlash Chromatin Isolation & Shearing Kit addresses the inconvenience and time consuming issues of existing chromatin preparation methods by introducing the following features:
• Fast procedure: the entire procedure from cell/tissue sample to ready-to-use sheared chromatin is less than 2 hours.
• Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues.
• Chromatin shearing step is pre-optimized for sonication with various sonication platforms including water bath-based (EpiSonic, Covaris, Bioruptor) sonicators, probe-based sonicators (Branson, Misonix), and enzyme-based methods.