A complete set of optimized reagents to enrich and capture methylated DNA fragments in a convenient microplate-based format.
The method, methylated DNA immunoprecipitation (meDIP), uses a monoclonal antibody specific to 5-methylcytosine to immunoprecipitate methylated genomic DNA. The enriched methylated fractions can then be used for gene-specific DNA methylation analysis on a genome wide scale. The Methylamp Methylated DNA Capture Kit is suitable for combining the specificity of enriched methylated DNA with qualitative and quantitative PCR, traditional sequencing and next-generation sequencing, DNA microarrays, as well as Southern blot.
The kit has the following advantages and features:
• Highly efficient enrichment of methylated DNA: > 98%.
• Extremely fast procedure, which can be finished within 3 hours.
• Strip-well microplate format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included, saving time and reducing labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.